loc
BLAST
-
local NCBI BLAST
Choose Program to Use and Database to Search
Program:
BlastN
tBlastN
Database:
All database
Anthonomus grandis
Diatraea saccharalis
Helicoverpa armigera
Hypothenemus hampei
Spodoptera frugiperda
Telchin licus licus
Enter sequence below in
FASTA
format (
DEMO
)
Or load it from disk
Output:
Graphical Overview
Plain Text
Algorithm Parameters:
Default
Advanced
Maximum Target Sequences:
0
10
50
100
250
500
Expect Threshold:
0.0001
0.01
1.0
10.0
100.0
1000.0
Word Size:
11
20
24
28
32
48
64
128
256
Match/Mismatch Scores:
Match: 1, Mismatch: -2
Match: 1, Mismatch: -3
Match: 1, Mismatch: -4
Match: 2, Mismatch: -3
Match: 4, Mismatch: -5
Match: 1, Mismatch: -1
Gap Costs:
Linear
Existence: 5, Extension: 2
Existence: 2, Extension: 2
Existence: 1, Extension: 2
Existence: 0, Extension: 2
Existence: 3, Extension: 1
Existence: 2, Extension: 1
Existence: 1, Extension: 1
Filter Low Complexity Regions
Mask for Lookup Table Only
Maximum Target Sequences:
0
10
50
100
250
500
Expect Threshold:
0.0001
0.01
1.0
10.0
100.0
1000.0
Word Size:
6
11
20
24
28
32
48
64
128
256
Matrix:
PAM30
PAM70
PAM250
BLOSUM80
BLOSUM62
BLOSUM45
BLOSUM50
BLOSUM90
Gap Costs:
Existence: 11, Extension: 2
Existence: 10, Extension: 2
Existence: 9, Extension: 2
Existence: 8, Extension: 2
Existence: 7, Extension: 2
Existence: 6, Extension: 2
Existence: 13, Extension: 1
Existence: 12, Extension: 1
Existence: 11, Extension: 1
Existence: 10, Extension: 1
Existence: 9, Extension: 1
Filter Low Complexity Regions
Mask for Lookup Table Only
Maximum Target Sequences:
0
10
50
100
250
500
Expect Threshold:
0.0001
0.01
1.0
10.0
100.0
1000.0
Word Size:
6
11
20
24
28
32
48
64
128
256
Matrix:
PAM30
PAM70
PAM250
BLOSUM80
BLOSUM62
BLOSUM45
BLOSUM50
BLOSUM90
Gap Costs:
Existence: 11, Extension: 2
Existence: 10, Extension: 2
Existence: 9, Extension: 2
Existence: 8, Extension: 2
Existence: 7, Extension: 2
Existence: 6, Extension: 2
Existence: 13, Extension: 1
Existence: 12, Extension: 1
Existence: 11, Extension: 1
Existence: 10, Extension: 1
Existence: 9, Extension: 1
Filter Low Complexity Regions
Mask for Lookup Table Only
Maximum Target Sequences:
0
10
50
100
250
500
Expect Threshold:
0.0001
0.01
1.0
10.0
100.0
1000.0
Word Size:
6
11
20
24
28
32
48
64
128
256
Matrix:
PAM30
PAM70
PAM250
BLOSUM80
BLOSUM62
BLOSUM45
BLOSUM50
BLOSUM90
Gap Costs:
Existence: 11, Extension: 2
Existence: 10, Extension: 2
Existence: 9, Extension: 2
Existence: 8, Extension: 2
Existence: 7, Extension: 2
Existence: 6, Extension: 2
Existence: 13, Extension: 1
Existence: 12, Extension: 1
Existence: 11, Extension: 1
Existence: 10, Extension: 1
Existence: 9, Extension: 1
Filter Low Complexity Regions
Mask for Lookup Table Only
Maximum Target Sequences:
0
10
50
100
250
500
Expect Threshold:
0.0001
0.01
1.0
10.0
100.0
1000.0
Word Size:
2
3
Matrix:
PAM30
PAM70
PAM250
BLOSUM80
BLOSUM62
BLOSUM45
BLOSUM50
BLOSUM90
Strand:
Both
Plus
Minus
Mask for Lookup Table Only
Mask Lower Case Letters
Maximum Target Sequences:
0
10
50
100
250
500
Expect Threshold:
0.0001
0.01
1.0
10.0
100.0
1000.0
Word Size:
2
3
Matrix:
PAM30
PAM70
PAM250
BLOSUM80
BLOSUM62
BLOSUM45
BLOSUM50
BLOSUM90
Gap Costs:
Existence: 11, Extension: 2
Existence: 10, Extension: 2
Existence: 9, Extension: 2
Existence: 8, Extension: 2
Existence: 7, Extension: 2
Existence: 6, Extension: 2
Existence: 13, Extension: 1
Existence: 12, Extension: 1
Existence: 11, Extension: 1
Existence: 10, Extension: 1
Existence: 9, Extension: 1
DELTA-BLAST Threshold:
Pseudocount:
Maximum Target Sequences:
0
10
50
100
250
500
Expect Threshold:
0.0001
0.01
1.0
10.0
100.0
1000.0
Word Size:
2
3
Matrix:
PAM30
PAM70
PAM250
BLOSUM80
BLOSUM62
BLOSUM45
BLOSUM50
BLOSUM90
Gap Costs:
Existence: 11, Extension: 2
Existence: 10, Extension: 2
Existence: 9, Extension: 2
Existence: 8, Extension: 2
Existence: 7, Extension: 2
Existence: 6, Extension: 2
Existence: 13, Extension: 1
Existence: 12, Extension: 1
Existence: 11, Extension: 1
Existence: 10, Extension: 1
Existence: 9, Extension: 1
PSI-BLAST Threshold:
Pseudocount:
Maximum Target Sequences:
0
10
50
100
250
500
Maximum Descriptions:
0
10
50
100
250
500
Expect Threshold:
0.0001
0.01
1.0
10.0
100.0
1000.0
Filter Low Complexity Regions
Mask for Lookup Table Only
Mask Lower Case Letters
Maximum Target Sequences:
0
10
50
100
250
500
Maximum Descriptions:
0
10
50
100
250
500
Expect Threshold:
0.0001
0.01
1.0
10.0
100.0
1000.0
Strand:
Both
Plus
Minus
Filter Low Complexity Regions
Mask for Lookup Table Only
Mask Lower Case Letters